Journal: Frontiers in Veterinary Science
Article Title: Evaluation of the immunogenicity of a Crimean-Congo hemorrhagic fever virus vaccine candidate in mice developed based on a baculovirus Zera nanoparticle delivery system
doi: 10.3389/fvets.2023.1126785
Figure Lengend Snippet: Identification of recombinant plasmids by double digestion and bacmid PCR. (A) Zera-Gn-mCherry double digestion identification map, Lane 1: Zera-Gn-pFastBac Dual/Xho I + Sph I (1940 bp), Lane 2: plasmid; (B) Zera-Np-eGFP double digestion identification map, Lane 1: Zera-Np-pFastBac Dual/BamH I + Xba I (2,549 bp), Lane 2: plasmid; (C,D) Recombinant bacmid PCR identification. Lane 1: Bacmid-Zera-Np-eGFP PCR; Lane 2: Bacmid-Zera-Gn-mCherry PCR.
Article Snippet: The baculovirus expression vector pFastBac Dual (Invitrogen, United States) was provided by Prof. Yulong He from Zhejiang University of Technology.
Techniques: Recombinant, Plasmid Preparation